RIPK2 Antibody (C-term)

SKU: orb38573

Description

Rabbit polyclonal antibody to RIPK2

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Signaling Pathways,Signaling Pathways/Apoptotic,Immunology,Epigenetics,Primary Antibodies;Cancer;Cell Biology;Immunology;Metabolism;Signal Transduction;Anti-Kinome Antibodies;Immunity;Innate immunity

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:50-100
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB03318
• Immunogen: Synthetic Peptide
• Target: RIPK2
• Molecular Weight: 61195
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-"Receptor-interacting serine/threonine protein kinase 2 antibody, anti-RIP-like interacting CLARP kinase antibody, anti-Receptor-interacting protein 2 antibody, anti-RIP-2 antibody, anti-CARD-containing interleukin-1 beta converting enzyme associated kinase antibody, anti-CARD-containing IL-1 beta ICE-kinase" antibody, anti-RIP2 antibody

Western blot analysis of anti-RIPK2 Pab in Ramos cell line lysates (35 ug/lane). RIPK2 (arrow) was detected using the purified Pab.

Western blot analysis of RIPK2 (arrow) using rabbit polyclonal RIPK2 Antibody (D474). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RIPK2 gene.

Western blot analysis of anti-RIPK2 Pab in mouse cerebellum tissue lysate. RIPK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Anti-RIPK2 Antibody (D474) at 1:1000 dilution + Raji whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RIPK2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

RIPK2

UniProt

O43353

RefSeq (Protein):NP_003812.1