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Description
Images & Validation
−
| Tested Applications | ICC, IHC, IP, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000; IP: 1/20; IHC: 1/50-1/100; ICC: 1/50-1/200 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide of human RNA polymerase II CTD repeat YSPTSPS |
| Target | RNA Polymerase II Subunit B1 |
| Molecular Weight | Calculated MW: 217 kDa; Observed MW: 250 kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−POLR2A; POLR2; DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
IP
Immunoprecipitation























