RPS6 Antibody (N-term)

SKU: orb305841

Description

Mouse monoclonal antibody to RPS6

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Crown Antibodies;Primary Antibodies;Cell Biology;Ribonucleoprotein;Viral transcription

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: FACS: 1:25, IF/ICC: 1:25, IHC-P: 1:25, WB: 1:2000
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse, Rabbit

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 981CT2.1.1
• Immunogen: Recombinant Protein
• Target: RPS6
• Molecular Weight: 28681
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti 40S ribosomal protein S6 antibody, anti Phosphoprotein NP33 antibody, anti RPS6 antibody

Western blot analysis of lysate from 293 cell line, using RPS6 Antibody (N-term). Diluted at 1:1000. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysate at 35 μg.

Immunohistochemical analysis of paraffin-embedded H. brain section using RPS6 Antibody (N-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. kidney section using RPS6 Antibody (N-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. kidney section using RPS6 Antibody (N-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Fluorescent image of Hela cells stained with RPS6 Antibody (N-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Fluorescent image of Hela cells stained with RPS6 Antibody (N-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Western blot analysis of lysates from 293, Hela, mouse NIH/3T3, rat PC-12 cell line and rat brain tissue lysate (from left to right), using RPS6 Antibody (N-term). Diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysates at 35 μg per lane.

Quick Database Links

Gene Symbol

RPS6

UniProt

P62753