RXR beta Antibody / RXRB

SKU: orb2636835

Description

Two families of retinoid receptors, RARs and RXRs, have been identified. Retinoic acid receptors (RARs) include RAR, are activated by 9-cis-RA, a stereo- and photo-isomer of all trans-RA that is expressed in vivo in both liver and kidney and may represent a widely used hormone. As is true for the RAR subfamily, the RXR receptors are closely related to each other both in their DNA-binding and ligand-binding domains and are encoded by separate genes at distinct chromosomal loci.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the RXRB antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: PCRP-RXRB-2B6
• Immunogen: Recombinant full-length human RXR beta protein was used as the immunogen for the RXRB antibody.
• Purity: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Hazard Information: This RXRB antibody is available for research use only.
• Disclaimer: For research use only

Immunofluorescent staining of PFA-fixed human K562 cells using RXRB antibody (green, clone PCRP-RXRB-2B6) and phalloidin (red).

Immunofluorescent staining of PFA-fixed human HeLa cells using RXRB antibody (green, clone PCRP-RXRB-2B6) and phalloidin (red).

IHC staining of FFPE human lymph node tissue with RXRB antibody (clone PCRP-RXRB-2B6). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

FACS staining of PFA-fixed human U-87 cells with RXRB antibody (blue, clone PCRP-RXRB-2B6), and unstained cells (red).

FACS staining of PFA-fixed human HeLa cells with RXRB antibody (blue, clone PCRP-RXRB-2B6), and unstained cells (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using RXRB antibody (clone PCRP-RXRB-2B6). These results demonstrate the foremost specificity of the PCRP-RXRB-2B6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free RXRB antibody (clone PCRP-RXRB-2B6) as confirmation of integrity and purity.

Quick Database Links

UniProt

P28702