S100A4 Antibody, KO Validated

SKU: orb1274487

Description

S100A4 Antibody, KO Validated

Research Area

,Cancer,Cell Cycle,Neuroscience,Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human S100A4 (NP_002952.1).
• Target: S100A4
• Molecular Weight: Observed: 12kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

S100A4, S100 calcium binding protein A4, 18A2, 42A, CAPL, MTS1, P9KA, PEL98, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium-binding protein A4, S100 calcium-binding protein A4 (calcium protein, malignant transformation suppression 1

Western blot analysis of extracts of various cell lines, using S100A4 antibody (orb1274487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Western blot analysis of extracts from normal (control) and S100A4 knockout (KO) HeLa cells, using S100A4 antibody (orb1274487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Immunohistochemistry of paraffin-embedded rat brain using S100A4 antibody (orb1274487) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using S100A4 antibody (orb1274487) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using S100A4 antibody (orb1274487) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using S100A4 antibody (orb1274487) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using S100A4 antibody (orb1274487) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of RAW264.7 cells using S100A4 antibody (orb1274487) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

S100A4

UniProt

P26447