Sample Diluent Optimization Pack

SKU: orb1409889

Description

ICT’s Sample Diluent Optimization Pack provides four 100 mL bottles of Sample Diluent formulations (General Serum Diluent, Plasma Sample Diluent, Neptune Sample Diluent, and Protein-Free Sample Diluent) for an economical and fast method to screen for the best Sample Diluent for an assay.

Research Area

,Product Categories/Tools

Images & Validation

• Application Notes:
  1. Prepare samples and controls., 2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20., 3. Reconstitute FLICA with 50 μL DMSO., 4. Dilute FLICA 1:5 by adding 200 μL PBS., 5. Add diluted FLICA to each sample at 1:30 – 1:60. For example, to stain at 1:30, add 10 μL to 290 μL of cultured cells. To stain at 1:60, add 5 μL to 295 μL of cultured cells., 6. Incubate approximately 1 hour., 7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buff er and spin cells., 8. If desired, label with additional stains, such as Hoechst, 7-AAD, or an antibody., 9. If desired, fix or embed cells., 10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. SR-FLICA excites at 550-580 nm and emits at 590-600 nm.

Key Properties

• Concentration: 1X

Storage & Handling

• Storage: 2-8°C
• Expiration Date: Please enquire.
• Disclaimer: For research use only