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Description
Research Area
,Recombinant-Protein
Images & Validation
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| Application Notes |
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Key Properties
−| Expression System | HEK293 Cells |
|---|---|
| Biological Origin | SARS-CoV-2 |
| Biological Activity | SARS-CoV-2 (B.1.214.2) Spike RBD Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.56 kDa and the accession number is YP_009724390.1. |
| Tag | C-His |
| Expression Region | A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutation Q414K, N450K) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.214.2). Predicted N terminal: Arg 319 |
| MW | 26.56 kDa (predicted); 34.36 kDa (reducing condition, due to glycosylation) |
| Purity | 98.00% |
Storage & Handling
−| Storage | -20°C |
|---|---|
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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