SARS-CoV-2 (BA.4/BA.5/BA.5.2) Spike S1+S2 trimer Protein (His & Avi), Biotinylated

SKU: orb1953566

Description

SARS-CoV-2 (BA.4/BA.5/BA.5.2) Spike S1+S2 trimer Protein (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 138.07 kDa and the accession number is YP_009724390.1.

Research Area

,Recombinant-Protein

Images & Validation

• Application Notes:
  A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

Key Properties

• Expression System: HEK293 Cells
• Biological Origin: SARS-CoV-2
• Biological Activity: SARS-CoV-2 (BA.4/BA.5/BA.5.2) Spike S1+S2 trimer Protein (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 138.07 kDa and the accession number is YP_009724390.1.
• Tag: C-His-Avi
• Expression Region: A DNA sequence encoding the SARS-CoV-2 (BA.4/BA.5/BA.5.2) Spike S1+S2 trimer (YP_009724390.1, with mutations T19I, L24del, P25del, P26del, A27S, H69del, V70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, F817P, A892P, A899P, A942P, Q954H, N969K, K986P, V987P and furin cleavage site mutants) (Met1-1208Q) was expressed with bacteriophage T4 fibritin and a C-terminal polyhistidine tag followed by an AVI tag. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.4/BA.5/BA.5.2).The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Val 16
• MW: 138.07 kDa (predicted)
• Purity: 98.00%

Storage & Handling

• Storage: -20°C
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

YP_009724390.1