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SARS-COV-2 BQ.1.1 (Omicron) Spike RBD Protein (His & Avi), Biotinylated
SKU: orb1953307
Description
Research Area
,Recombinant-Protein
Images & Validation
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| Application Notes |
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Key Properties
−| Expression System | HEK293 Cells |
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| Biological Origin | SARS-CoV-2 |
| Biological Activity | SARS-COV-2 BQ.1.1 (Omicron) Spike RBD Protein (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 26.58 kDa and the accession number is YP_009724390.1. |
| Tag | C-His-Avi |
| Expression Region | A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutation G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, K444T, L452R, N460K, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H) (Arg319-Phe541) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. The mutations were identified in the SARS-CoV-2 variant (known as variant BQ.1.1). Predicted N terminal: Val 16 |
| MW | 26.58 kDa (predicted); 35.29 kDa (reducing condition, due to glycosylation) |
| Purity | 98.00% |
Storage & Handling
−| Storage | -20°C |
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| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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