SARS-CoV-2 (COVID-19) Membrane Antibody (HRP)

SKU: orb1239083

Description

SARS-CoV-2 (COVID-19) Membrane Antibody (HRP)

Research Area

,Infectious Disease,COVID-19

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Virus
• Application Notes:
  IHC: 2 μg/mL Antibody validated: Immunohistochemistry in COVID-19 patient samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Membrane antibody (HRP) (9157) was raised against a peptide corresponding to 13 amino acids near the center of SARS-CoV-2 (COVID-19) Membrane protein. The immunogen is located between 130-180 amino acids of the SARS-CoV-2 (COVID-19) Membrane protein.
• Target: M
• Purification: SARS-CoV-2 (COVID-19) Membrane Antibody is affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: SARS-CoV-2°COVID-19) Membrane antibody can be stored at 4 ˚ C for three months and -20 ˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Form/Appearance: Liquid
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) Membrane Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SARS-CoV-2/SARS-CoV Matrix Antibody: SARS-CoV-2 Matrix protein, SARS Matrix protein, Membrane protein, E1 glycoprotein, M protein

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Membrane Protein in COVID-19 Patient Lung.Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) membrane antibody (orb1239066, 2 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. Counter stained with Hematoxylin. SARS-COV-2 membrane protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.

ELISA Validation.Coating Antigen: immunogen peptide, orb1231692, 10 μg/mL, incubate at 4°C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, orb1239083 or orb1239084, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. orb1239084 was detected by anti-rabbit HRP-conjugated secondary antibodies at 1:10, 000, incubate at RT for 1 hr.

Quick Database Links

Gene Symbol

M

UniProt

P0DTC5

RefSeq:QJW69073