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SARS-CoV (Isolate Tor2) Spike RBD Protein (His & Avi), Biotinylated
SKU: orb1953733
Description
Research Area
,Recombinant-Protein
Images & Validation
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| Application Notes |
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Key Properties
−| Expression System | HEK293 Cells |
|---|---|
| Biological Origin | SARS |
| Biological Activity | SARS-CoV (Isolate Tor2) Spike RBD Protein (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 28.4 kDa and the accession number is NP_828851.1. |
| Tag | His, Avi |
| Expression Region | A DNA sequence encoding the SARS-CoV (isolate Tor2) spike RBD (NP_828851.1) (Arg306-Phe527) was expressed with a c-terminal AVI tagged polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Ser |
| MW | 28.4 kDa (predicted); 37.1 kDa (reducing condition, due to glycosylation) |
| Purity | 98.00% |
Storage & Handling
−| Storage | -20°C |
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| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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