SARS S Protein, His Tag

SKU: orb750333

Description

SARS S protein (R667A, K968P, V969P), His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A, K968P, V969P)). The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively.

Research Area

Infectious Disease & Virology

Images & Validation

−

Application Notes	This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 136.3 kDa. The protein migrates as 190-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

−

Expression System	HEK293
Biological Origin	SARS
Target	Spike protein
Reactivity	Virus
Tag	C-10×His
Expression Region	Ser 14 - Pro 1195
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated
MW	136.3 kDa
Purity	95%
Protein Sequence	AAP13567.1 (R667A, K968P, V969P)

Storage & Handling

−

Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	50 mM Tris, 150 mM NaCl, 0.2 M Sodium citrate, pH 5.5
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

SARS-CoV-2 (2019-nCoV) S protein RBD, His Tag [orb689397]

Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 37-38 kDa after removal of the signal peptide.
Source:	Mammalian

Sizes Available: 100 μg, 10 μg, 50 μg

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

Gene Symbol

Spike protein

RefSeq:V969, AAP13567.1, K968, R667

NCBI Reference Sequences

−

Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	V969, AAP13567.1, K968, R667

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Protein Handling and Storage Guide

Protein Handling Guide

View Guide

Find More Protocols