SELL Antibody

SKU: orb1271024

Description

SELL Antibody

Research Area

,Immunology,Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Reactivity: Human
• Application Notes:
  For FACS starting dilution is: 1:25For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This SELL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-372 amino acids from the C-terminal region of human SELL.
• Target: SELL
• Molecular Weight: 42 kDa
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, LAM-1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, LECAM1, Lymph node homing receptor, TQ1, gp90-MEL, CD62L, SELL, LNHR, LYAM1

Overlay histogram showing Jurkat cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Western Blot at 1:2000 dilution Lane 1: Raji whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.

Formalin-fixed and paraffin-embedded human lymph tissue with SELL Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Quick Database Links

Gene Symbol

SELL

UniProt

P14151

RefSeq:P14151