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SERPINB5/Maspin Antibody

SKU: orb1539006

Description

SERPINB5/Maspin Antibody

Research Area

Product Categories/Products/Antibodies

Images & Validation

Tested ApplicationsELISA, IHC-P, WB
Dilution RangeELISA (0.5 - 2 µg/ml), IHC-P (2.5 - 5 µg/ml), WB (0.1 - 0.2 µg/ml)
ReactivityHuman
Application Notes
Resuspension: Centrifuge the vial before opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex. . Further information: Immunohistochemistry: LS-B7484 in an antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 2.5 ug/ml is recommended. ELISA: To detect hMaspin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Maspin (LS-C104540) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMaspin. Western Blot: To detect hMaspin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMaspin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Key Properties

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenE.coli derived recombinant Human Maspin.
TargetSERPINB5 / Maspin
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLyophilized from PBS, pH 7.2. Lyophilized from PBS, pH 7.2
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

SERPINB5, Maspin, Peptidase inhibitor 5, PI5, Protease inhibitor 5 (maspin), Serpin B5, PI-5

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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50 μg
$ 670.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-3 weeks
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