Serum Response Factor Antibody / SRF

SKU: orb2634817

Description

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1), have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino-terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF. The third homologous region, the C box located toward the C-terminus of the proteins, contains conserved consensus phosphorylation sites for MAP kinases.

Images & Validation

• Tested Applications: ELISA, FACS, IF, IHC-P, WB
• Dilution Range: ELISA (order BSA-free format for coating),Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Serum Response Factor antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: PCRP-SRF-1F7
• Immunogen: Recombinant full-length human protein was used as the immunogen for the Serum Response Factor antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Serum Response Factor antibody is available for research use only.
• Disclaimer: For research use only

Quick Database Links

UniProt

P11831