Sheep IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed

SKU: orb347921

Description

Sheep IgG (H&L) antibody (FITC)

Research Area

Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: FC, FLISA, IF, WB
• Dilution Range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Reactivity: Sheep
• Application Notes:
  Anti-Sheep IgG Fluorescein Conjugated Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Sheep IgG whole molecule
• Purity: Sheep IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Proteins.
• Conjugation: FITC

Storage & Handling

• Storage: Store Secondary Antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-Sheep IgG fluorescein Conjugated Antibody, Rabbit Anti-Sheep IgG Antibody FITC Conjugation