SI-CLP Antibody / CHID1 / Chitinase Domain Containing 1

SKU: orb1826112

Description

SI-CLP (stabilin-1 interacting chitinase-like), also known as CHID1 (chitinase domain-containing protein 1), is a 393 amino acid secreted protein that belongs to the glycosyl hydrolase 18 family. SI-CLP interacts with STAB1 and localizes to lysosome in cells of monocytic, T, B and epithelial origin. SI-CLP is a saccharide- and LPS-binding protein with possible roles in pathogen sensing and endotoxin neutralization. Ligand-binding specificity relates to the length of the oligosaccharides, with preference for chitotetraose (in vitro). The SI-CLP protein is up-regulated by IL4/interleukin-4 and dexamethasone in the macrophages, and is also up-regulated by glucocorticoid. Existing as three alternatively spliced isoforms, the SI-CLP gene is conserved in chimpanzee, canine, bovine, mouse, rat, chicken, zebrafish, fruit fly, mosquito, A. thaliana and rice, and maps to human chromosome 11p15.5.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: CHID1/6368
• Immunogen: Recombinant full-length human SI-CLP protein was used as the immunogen for the CHID1 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the SI-CLP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This SI-CLP antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human prostate tissue with SI-CLP antibody (clone CHID1/6368) HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human esophagus tissue with SI-CLP antibody (clone CHID1/6368) Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

Q9BWS9