Siah-1/2 rabbit pAb

SKU: orb766317

Description

Rabbit polyclonal antibody to Siah-1/2

Research Area

Product Categories/Antibodies/Primary Antibodies,Product Categories/Small Molecules,Product Categories/Antibodies

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution Range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human SIAH1. AA range:181-230
• Molecular Weight: 34
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-SIAH1 antibody, anti-HUMSIAH antibody, anti-E3 ubiquitin-protein ligase SIAH1 antibody, anti-Seven in absentia homolog 1 antibody, anti-Siah-1 antibody, anti-Siah-1a antibody, anti-SIAH2 antibody, anti-E3 ubiquitin-protein ligase SIAH2 antibody, anti-Seven in absentia homolog 2 antibody, anti-Siah-2 antibody, anti-hSiah2 antibody

Western Blot analysis of various cells using Siah-1/2 Polyclonal Antibody diluted at 1:500.

Western Blot analysis of HeLa cells using Siah-1/2 Polyclonal Antibody diluted at 1:500.

Western blot analysis of lysates from A549 and HeLa cells, using SIAH1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).