SIRT1 Antibody, KO Validated

SKU: orb1274544

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Description

SIRT1 Antibody, KO Validated

Research Area

,Apoptosis,Cell Cycle

Images & Validation

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Item 1 of 6

Tested Applications	IF, IHC, IP, WB
Reactivity	Human, Mouse, Rat
Application Notes	WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Key Properties

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Antibody Type	Primary Antibody
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 448-747 of human SIRT1 (NP_036370.2).
Target	SIRT1
Molecular Weight	Observed: 140kDa
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid
Concentration	batch dependent
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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SIRT1 Antibody: SIR2L1, SIR2L1, NAD-dependent protein deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, hSIRT1

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western blot analysis of extracts of various cell lines, using SIRT1 antibody (orb1274544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts from normal (control) and SIRT1 knockout (KO) HeLa cells, using SIRT1 antibody (orb1274544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human mammary cancer using SIRT1 antibody (orb1274544) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using SIRT1 antibody (orb1274544) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using SIRT1 antibody (orb1274544) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of Jurkat cells, using 3 ug SIRT1 antibody (orb1274544). Western blot was performed from the immunoprecipitate using SIRT1 antibody (orb1274544) at a dilution of 1:1000.