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SKA3 Antibody

SKU: orb1249462

Description

SKA3 Antibody

Research Area

,Cell Cycle

Images & Validation

Tested ApplicationsELISA, IHC
ReactivityHuman
Predicted ReactivityCanine, Mouse, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 35kDa and 150kDa band in HeLa lysates after 2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 46.4kDa according to NP_659498.3. Both bands were successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 3.75ug/ml.

Key Properties

Antibody TypePrimary Antibody
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-PLSKTNSSSNDLE
TargetC13orf3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

C13orf3, chromosome 13 open reading frame 3, MGC4832, RAMA1, SKA3

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 880.00
Free Secondary Antibody (20 ul)0/0

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