SLC26A4 Rabbit Polyclonal Antibody

SKU: orb101538

Description

SLC26A4 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=1ug/test, ELISA=1:5000-10000
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Solute carrier family 26 member 4 (301-400/780aa)
• Target: SLC26A4
• Molecular Weight: 93 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PDS; deafness, autosomal recessive 4; DFNB4; EVA; NSRD4; Pendred syndrome; Pendred syndrome homolog; Pendrin; S26A4_HUMAN; SLC26A4; Sodium independent chloride/iodide transporter; Sodium-independent chloride/iodide transporter; Solute carrier family 26 member 4.

Black line: Positive blank control (U87MG), Negative blank control (Molt4), Green line: Primary Antibody (Rabbit Anti-SLC26A4 antibody (orb101538)), Orange line:Isotype Control Antibody (Rabbit IgG). Blue line: Secondary Antibody (Goat anti-rabbit IgG-PE)/ (Goat anti-rabbit IgG-AF488), U87MG (Positive) and Molt4 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SLC26A4 Antibody (orb101538) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Blank control: U87MG. Primary Antibody (green line): Rabbit Anti-SLC26A4 antibody (orb101538) Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 1 µg/test. The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Human TT cell lysates, Lane 2: Human U251 cell lysates, Primary: Anti-SLC26A4 (orb101538) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 93 kDa, Observed band size: 93 kDa.

Quick Database Links

Gene Symbol

SLC26A4

UniProt

O43511