SLUG Antibody (Center)

SKU: orb1931752

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Primary Antibodies;Cancer;Cardiovascular;Developmental Biology;Neuroscience;Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF: 1:10~50, WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB01398
• Target: This SLUG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-129 amino acids from the Central region of human SLUG.
• Molecular Weight: 29986 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Zinc finger protein SNAI2, Neural crest transcription factor Slug, Protein snail homolog 2, SNAI2, SLUG, SLUGH

Confocal immunofluorescent analysis of SLUG Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human Breast tissue reacted with SLUG Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

SLUG Antibody (Center) is used in Western blot to detect SLUG in mouse heart tissue lysate.

All lanes: Anti-SLUG Antibody (Center) at 1:2000 dilution. Lane 1: mouse heart lysate. Lane 2: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 30 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This SLUG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-129 amino acids from the Central region of human SLUG.

UniProt

O43623

RefSeq (Protein):NP_003059.1