SMARCA4 Rabbit Polyclonal Antibody

SKU: orb704395

Description

SMARCA4 Rabbit Polyclonal Antibody

Research Area

Cell Markers, Neuroscience, Nuclear Signaling Pathway, SWI/SNF (SWItch/Sucrose Non-Fermentable), Cancer Research, Trithorax-group proteins (TrxG), Tumor Suppressors, Cancer Research

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:50-100, IHC-F=1:50-100, ICC/IF=1:100, IF=1:20-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SMARCA4 (30-130/1647aa)
• Target: SMARCA4
• Molecular Weight: 181 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SMCA4_HUMAN; Transcription activator BRG1; EC:3.6.4.-; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A(BAF190A); Mitotic growth and transcription activator; Protein BRG-1; Protein brahma homolog 1; BAF190A; BRG1; SNF2B; SNF2L4; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4; CSS4; SNF2; SWI2; MRD16; RTPS2; BAF190; OTSC12; SNF2LB; hSNF2b; SNF2-beta;

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (KO Validated) SMARCA4 polyclonal Antibody, Unconjugated (orb704395) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SMARCA4) Polyclonal Antibody, Unconjugated (orb704395) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SMARCA4) Polyclonal Antibody, Unconjugated (orb704395) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: 293T (Human) Cell Lysate at 25 ug, Lane 2: SMARCA4 knockout (KO) 293T (Human) Cell Lysate at 25 ug, Primary: Anti-SMARCA4 (orb704395) at 1/500 dilution, Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution, Predicted band size: 180 kD, Observed band size: 180 kD.

Quick Database Links

Gene Symbol

SMARCA4

UniProt

P51532