SOD2 Antibody

SKU: orb167070

Description

Mouse monoclonal antibody to SOD2.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cardiovascular Disease,Neuroscience,Epigenetics,Crown Antibodies;Primary Antibodies;Cancer;Cardiovascular;Cell Biology;Metabolism;Neuroscience;Signal Transduction;Mitochondrion

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:2000, IHC-P: 1:10-50
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1κ
• Clone No.: 37CT127.5.11.6
• Immunogen: Recombinant Protein
• Target: SOD2
• Molecular Weight: 24750
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti SOD2 antibody, anti Superoxide dismutase [Mn], mitochondrial antibody

All lanes: Anti-SD Antibody at 1:2000 dilution. Lane 1: Hela whole cell lysates. Lane 2: human brain lysates. Lane 3: human heart lysates. Lane 4: human liver lysates.Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOD2 Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-SOD2 Monoclonal Antibody by SOD2-GST fusion protein (GST MW=26 kD. SOD2-GST fusion protein (arrow) was detected using the purified Mab. (1:2000)

Quick Database Links

Gene Symbol

SOD2

UniProt

P04179

RefSeq (Protein):NP_001019636.1, NP_001019637.1, NP_000627.2

Bertolo, Alessandro et al. Oxidative status predicts quality in human mesenchymal stem cells Stem Cell Res Ther, 8, 3 (2017)

PubMed: 28061861

Thomas-Jardin, Shayna E. et al. Identification of an IL-1-induced gene expression pattern in AR+ PCa cells that mimics the molecular phenotype of AR- PCa cells Prostate, 78, 595-606 (2018)

PubMed: 29527701