SQSTM1 / P62

SKU: orb1294360

Description

Rabbit polyclonal antibody to SQSTM1

Research Area

,Product Categories/Antibodies,Autophagy

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:1000-1:2000 Immunofluorescence 1:100-1:400 Immunohistochemistry (Paraffin) 1: 100-1:200
• Reactivity: Human, Mouse, Rabbit

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the N-terminus region.
• Target: SQSTM1 / P62
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-SQSTM1/p62 antibody - at 1/1000 dilution. Lysates at 35 μg per lane. This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294360 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling SQSTM1/p62 with orb1294360 at 1/200.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294360 Anti-SQSTM1/p62 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling SQS-TM1/p62 with orb1294360 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling SQS-TM1/p62 with orb1294360 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294360 Anti-SQSTM1/p62 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294360 Anti-SQSTM1/p62 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294360 Anti-SQSTM1/p62 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded mouse kidney labeling SQS/TM1/P62 with orb1294360 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse spleen labeling SQS/TM1/P62 with orb1294360 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294360 Anti-SQSTM1/p62 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Quick Database Links

Gene Symbol

SQSTM1 / P62