sRANK Ligand Antibody (biotin)

SKU: orb1272305

Description

sRANK Ligand Antibody (biotin)

Research Area

,Chemokines & Cytokines

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  ELISA:Sandwich:To detect hsRANKL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human sRANKL (XP-5274) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsRANKL. Western Blot:To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsRANKL (Human sRANK-Ligand).
• Target: TNFSF11
• Purification: Anti-hsRANKL specific antibody was purified by affinity chromatography and then biotinylated.
• Conjugation: Unconjugated

Storage & Handling

• Storage: sRANK Ligand antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-8˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRANKL2, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF

Quick Database Links

Gene Symbol

TNFSF11

UniProt

O14788

RefSeq:O14788