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Description
Research Area
Images & Validation
−| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:1000-1:2000 Immunofluorescence 1:300-1:600 Immunohistochemistry (Paraffin) 1:100-1:200 |
| Reactivity | Human, Mouse, Rabbit |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the center region |
| Target | ST3GAL1 / SIAT4A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |

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Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ST3GAL1 with orb1294261 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ST3GAL1 with orb1294261 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ST3GAL1 with orb1294261 at 1/100.

Anti-ST3GAL1 antibody - at 1/1000 dilution. Lysates at 35 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294261 overnight at 4°C.

Immunohistochemical of frozen sections. Red: ST3GAL1 (orb1294261): 1-200. Anti-rabbit 594: 1-500.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ST3GAL1 with orb1294261 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ST3GAL1 with orb1294261 at 1/100.

Anti-ST3GAL1 antibody - at 1/1000 dilution, Lysates/proteins at 35 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294261 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ST3GAL1 with orb1294261 at 1/100.
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Protocol Information
ST3GAL1 / SIAT4A (orb1294261)
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