STAT3

SKU: orb1294257

Description

Rabbit polyclonal antibody to STAT3

Research Area

,Product Categories/Antibodies,Signaling

Images & Validation

• Tested Applications: WB
• Dilution Range: Western Blot 1:1000-1:3000 Immunofluorescence 1:300-1:500
• Reactivity: Human, Mouse, Rabbit

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region
• Target: STAT3
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-STAT3 antibody at 1/2000 dilution, Lysates/proteins at 30 μg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294257 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294257 anti-STAT3 antibody (red) at 1:500 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Quick Database Links

Gene Symbol

STAT3