STAT3 Rabbit Polyclonal Antibody

SKU: orb11431

Description

STAT3 Rabbit Polyclonal Antibody

Research Area

Cardiovascular Research, Intercellular Signaling, Cell Signaling, JAK/ STAT Signaling Pathway, SARS /COVID-19, Surface Molecules, Molecular Biology, Transcription Factors, DNA and RNA Research

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=3μg/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Guinea pig, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human STAT3 (233-330/770aa)
• Target: STAT3
• Molecular Weight: 88 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

1110034C02Rik; Acute Phase Response Factor; APRF; AW109958; DNA binding protein APRF; FLJ20882; HIES; MGC128731; MGC16063; MGC93551; Signal transducer and activator of transcription 3(acute-phase response factor); Signal transducer and activator of transcription 3; Signal Transductor and Activator of Transcription 3; STAT 3; Transcription factor; STAT3_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Mouse lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (STAT3) Polyclonal Antibody, Unconjugated (orb11431) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Positive control: RSC96, Isotype Control Antibody: Rabbit IgG, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:200 in 1 X PBS containing 0.5% BSA, Primary Antibody catalog number: orb11431, Dilution: 3 µg in 100 µl 1X PBS containing 0.5% BSA.

Tissue/Cell: A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (STAT3) polyclonal Antibody, Unconjugated (orb11431) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

STAT3

UniProt

P40763