Stattic

SKU: orb1300882

Description

Stattic

Research Area

Product Categories/Products/Small Molecules,Apoptosis|||Stem Cells|||JAK/STAT signaling

Key Properties

• CAS Number: 19983-44-9
• MW: 211.19
• Purity: 99.56%
• Formula: C₈H₅NO₄S
• SMILES: [O-][N+](=O)C1=CC=C2C=CS(=O)(=O)C2=C1

Bioactivity

• Target IC50:
  DU-145 cells proliferation:2.5 μM (48h)|STAT3:5.1 μM (cell free)|A549 cells proliferation:4.4 μM (72h)|A549 cells proliferation:2.5 μM (48h)|ASPC1 cells proliferation:1.32 μM (72h)|HCT116 cells proliferation:1.08 μM (72h)
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• In Vivo:
  METHODS: To test the antitumor activity in vivo, Stattic (10 mg/kg) was administered intraperitoneally once daily for four weeks to BALB/c nude mice harboring human pancreatic adenocarcinoma tumor PANC-1. RESULTS: Stattic inhibited PC growth in a nude mouse tumor model by inactivating STAT3. METHODS: To investigate the role in acute liver injury, Stattic (5 mg/kg in DMSO:olive oil = 1:19) was administered as a single intraperitoneal injection to BALB/c mice with LPS/d-GalN induced acute liver injury. RESULTS: Stattic was protective against LPS/d-GalN-induced liver injury, and its protective effect may be related to its anti-inflammatory and anti-apoptotic effects.
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• In Vitro:
  METHODS: Human pancreatic cancer cells PANC-1 and BxPc-3 were treated with Stattic (1-10 μM) for 12-48 h. Cell viability was measured using the CCK-8. RESULTS: Stattic decreased the proliferation of PANC-1 and BxPc-3 cells in a concentration- and time-dependent manner, and the IC50s of Stattic on BxPc-3 and PANC-1 cells were 3.135-5.296 μM and 3.835-4.165 μM, respectively, after treatment with Stattic for 24 h. METHODS: Human hepatocellular carcinoma cells HepG2 were treated with Stattic (5-20 μM) for 1 h, followed by stimulation with IL-6 or IFN-γ, and the expression levels of target proteins were detected by Western Blot. RESULTS: Pre-incubation with Stattic resulted in a selective decrease in the phosphorylation of STAT3 Tyr705, while the activation of STAT1 Tyr701 remained unchanged.
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• Cell Research:
  MDA-MB-231, MDA-MB-435S, and MDA-MB-453 cells were seeded. at 5 × 10^4 cells in 6-well plates, grown for 24 hr before adding DMSO or Stattic (final DMSO concentration 0.1%) and then incubated with the inhibitor for 24 hr. All cells were collected and resuspended in buffer (0.1% sodium citrate, 0.1% Triton X-100, 20 μM propidium iodide) and incubated for 3 hr before 10^4 cells per sample were analyzed by flow cytometry with a FACSCalibur equipped with a 488 nm laser .
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Compound Identifiers

PubChem CID

2779853