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STEAP1/STEAP Antibody

SKU: orb1537960

Description

STEAP1/STEAP Antibody

Research Area

Product Categories/Products/Antibodies

Images & Validation

Tested ApplicationsELISA, ICC, IF, IHC, IHC-P, WB
Dilution RangeELISA, ICC, IF, IHC, IHC-P (10 µg/ml), WB (1:500 - 1:1000)
ReactivityHuman
Application Notes
Further information: Immunohistochemistry: LS-B3003 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3003 was determined to be 10 ug/ml.

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG2b,k
Clone No.J2D2
ImmunogenRecombinant human STEAP1 (1-70aa) purified from E. coli. NCBI Accession No: NP_036581.
TargetSTEAP1 / STEAP
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol. PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

STEAP1, Metalloreductase STEAP1, PRSS24, STEAP

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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50 μl
$ 640.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-3 weeks
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