Steroidogenic Factor 1 Antibody / SF-1 / NR5A1

SKU: orb1822700

Description

Steroidogenic Factor 1 (SF-1) is considered an orphan nuclear receptor that belongs to subfamily 5. It was found to be a regulator of steroidogenic enzyme gene expression. Oxysterols are suggested as its ligands. It is expressed in all steroidogenic tissues, including the adrenal cortex, testicular Sertoli cells, and Leydig cells, ovarian theca, hypothalamus, and anterior pituitary. SF-1 plays an important role in adrenal and gonadal development.SF-1 is highly valuable marker to determine the adrenocortical origin of an adrenal mass.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: SF1/8000R
• Immunogen: A recombinant partial protein sequence (within amino acids 200-400) from the human protein was used as the immunogen for the Steroidogenic Factor 1 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the Steroidogenic Factor 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Steroidogenic Factor 1 antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human adrenal cortex with Steroidogenic Factor 1 antibody (clone SF1/8000R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human testis tissue with Steroidogenic Factor 1 antibody (clone SF1/8000R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

Q13285