STIP1 monoclonal antibody (M01), clone 4B6

SKU: orb2291473

Description

Mouse monoclonal antibody raised against a full length recombinant STIP1.

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, IP, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a Kappa
• Clone No.: 4B6
• Immunogen: STIP1 (AAH02987.1, 1 a.a. ~ 543 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MEQVNELKEKGNKALSVGNIDDALQCYSEAIKLDPHNHVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKPDWGKGYSRKAAALEFLNRFEEAKRTYEEGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESDPRTRTLLSDPTYRELIEQLRNKPSDLGTKLQDPRIMTTLSVLLGVDLGSMDEEEEIATPPPPPPPKKETKPEPMEEDLPENKKQALKEKELGNDAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRELCEKAIEVGRENREDYRQIAKAYARIGNSYFKEEKYKDAIHFYNKSLAEHRTPDVLKKCQQAEKILKEQERLAYINPDLALEEKNKGNECFQKGDYPQAMKHYTEAIKRNPKDAKLYSNRAACYTKLLEFQLALKDCEECIQLEPTFIKGYTRKAAALEAMKDYTKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMRLILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Detection limit for recombinant GST tagged STIP1 is approximately 0.1 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.3 ug/ml]

Immunoprecipitation of STIP1 transfected lysate using anti-STIP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STIP1 MaxPab rabbit polyclonal antibody.

STIP1 monoclonal antibody (M01), clone 4B6 Western Blot analysis of STIP1 expression in HeLa.

Western Blot analysis of STIP1 expression in transfected 293T cell line by STIP1 monoclonal antibody (M01), clone 4B6. Lane 1: STIP1 transfected lysate(62.6 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (85.25 KDa).

Quick Database Links

RefSeq:AAH02987.1