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Description
Images & Validation
−| Tested Applications | ELISA, FC, IF, IHC-P, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 Kappa |
| Clone No. | 2E1 |
| Immunogen | STIP1 (AAH02987.1, 1 a.a. ~ 543 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Protein Sequence | MEQVNELKEKGNKALSVGNIDDALQCYSEAIKLDPHNHVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKPDWGKGYSRKAAALEFLNRFEEAKRTYEEGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESDPRTRTLLSDPTYRELIEQLRNKPSDLGTKLQDPRIMTTLSVLLGVDLGSMDEEEEIATPPPPPPPKKETKPEPMEEDLPENKKQALKEKELGNDAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRELCEKAIEVGRENREDYRQIAKAYARIGNSYFKEEKYKDAIHFYNKSLAEHRTPDVLKKCQQAEKILKEQERLAYINPDLALEEKNKGNECFQKGDYPQAMKHYTEAIKRNPKDAKLYSNRAACYTKLLEFQLALKDCEECIQLEPTFIKGYTRKAAALEAMKDYTKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMRLILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | In 1x PBS, pH 7.4 |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |

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Detection limit for recombinant GST tagged STIP1 is 0.1 ng/ml as a capture antibody.

FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained 293 cells (Black) as negative control.

FACS analysis of A-431 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained A-431 cells (Black) as negative control.

FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained ES-2 cells (Black) as negative control.

FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained HeLa cells (Black) as negative control.

FACS analysis of TOV-21G cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained TOV-21G cells (Black) as negative control.

Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 0.75 ug/ml]

Western Blot detection against Immunogen (85.25 KDa).
Quick Database Links
NCBI Reference Sequences
−| RefSeq | AAH02987.1 |
|---|
Documents Download
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Protocol Information
STIP1 monoclonal antibody (M33), clone 2E1 (orb2291469)
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