Streptavidin protein

SKU: orb82632

Description

Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1×10-15 M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin.

Images & Validation

• Tested Applications: FA, HPLC, SDS-PAGE
• Application Notes:
  Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Key Properties

• Biological Origin: E.coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 was determined by a cell proliferation assay using FDC-P1 cells is < 2.0 ng/ml, corresponding to a specific activity of > 5x10⁵ units/mg.
• Target: Streptavidin
• Endotoxins: Less than 0.1 EU/μg of rStreptavidin as determined by LAL method.
• Conjugation: Unconjugated
• MW: 7.6 kDa
• Purity: < p>> 98% pure by SDS-PAGE and HPLC analyses.

• Protein Sequence: MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS

Storage & Handling

• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized in 10 mM potassium phosphate buffer pH 6.5.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Streptavidin