SUMO1 Antibody

SKU: orb749835

Description

This mAb is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Infectious Diseases

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Reactivity: Human, Rat
• Application Notes:
  Optimal dilution of the SUMO-1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: SUMO1/1188
• Immunogen: Recombinant human protein was used as the immunogen for the SUMO-1 antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the SUMO-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This SUMO-1 antibody is available for research use only.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with SUMO-1 antibody (SUMO1/1188)

IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with SUMO-1 antibody (SUMO1/1188)

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with SUMO-1 antibody (SUMO1/1188)

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with SUMO-1 antibody (SUMO1/1188)

IHC: Formalin-fixed, paraffin-embedded rat pancreas stained with SUMO-1 antibody (SUMO1/1188)

Quick Database Links

UniProt

P63165