SUMO2/3 Antibody (N-term)

SKU: orb1937637

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Primary Antibodies;Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:1000, WB: 1:1000, WB: 1:2000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 10871 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Small ubiquitin-related modifier 2, SUMO-2, HSMT3, SMT3 homolog 2 {ECO:0000312|HGNC:HGNC:11125}, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B, Smt3B, SUMO2 (HGNC:11125)

All lanes: Anti-SUMO2/3 Antibody (M1) at 1:2000 dilution. Lane 1: A549 whole cell lysates. Lane 2: Hela whole cell lysates. Lane 3: HepG2 whole cell lysates. Lane 4: HL-60 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 11 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-SUMO2/3 N-term polyclonal antibody in 293 cell lysate. Sumo2/3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Western blot analysis of SUMO2/3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (M1). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2/3 gene.

Quick Database Links

UniProt

P61956

RefSeq (Protein):NP_008868.3, NP_001005849.1