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Synapsin (phospho Ser9) Antibody

SKU: orb1273512

Description

Synapsin (phospho Ser9) Antibody

Research Area

,Neuroscience,Phospho-Specific

Images & Validation

Tested ApplicationsIHC, WB
ReactivityFrog, Human, Mouse, Rat
Application Notes
Immunolabeling of the 78k Synapsin I doublet in a Western blot of rat brain homogenate is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. The antibody also weakly labels Synapsin II (55k) which has a similar phosphorylation site to that of Ser9 on Synapsin I. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Key Properties

Antibody TypePrimary Antibody
ClonalityPolyclonal
ImmunogenSynapsin (Ser9) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Ser9 of Rat Synapsin I.
TargetSYN1
Molecular Weight78
PurificationAffinity Purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP17599

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol

Available Sizes

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0.1 ml
$ 960.00
Free Secondary Antibody (20 ul)0/0

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