Synaptotagmin-7 Antibody

SKU: orb99093

Description

Mouse Anti-Mouse Synaptotagmin-7 Monoclonal IgG2B

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Neuroscience | Cell Markers | Neuron Markers | Presynaptic Markers

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-424 was sufficient for detection of Synaptotagmin-7 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2B
• Clone No.: N275/14 (Formerly sold as S275-14)
• Immunogen: Fusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7
• Target: Synaptotagmin 7
• Molecular Weight: 45kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IPCA-7 Antibody, PCANAP7 Antibody, Prostate cancer-associated protein 7 Antibody, SYT-7 Antibody, SYT7 Antibody, Synaptotagmin 7 Antibody, Synaptotagmin VII Antibody, SytVII Antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Synaptotagmin-7 Monoclonal Antibody, Clone N275/14. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Synaptotagmin-7 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Synaptotagmin-7 Antibody (D) Composite.

Western Blot analysis of Rat brain lysates showing detection of Synaptotagmin 7 protein using Mouse Anti-Synaptotagmin 7 Monoclonal Antibody, Clone N275/14. Primary Antibody: Mouse Anti-Synaptotagmin 7 Monoclonal Antibody at 1:100, 1:250, 1:500, and 1:1000.

Quick Database Links

Gene Symbol

Synaptotagmin 7

UniProt

Q9R0N7

NCBI Gene ID

54525

RefSeq (Protein):NP_061271.1