TAF4 Antibody (C-Term)

SKU: orb1925631

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Crown Antibodies;Primary Antibodies;Viral process;Host-virus interaction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:2000, IHC-P: 1:25, IHC-P: 1:25
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB56278
• Target: This TAF4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1021-1062 amino acids from human TAF4.
• Molecular Weight: 110114 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Transcription initiation factor TFIID subunit 4, RNA polymerase II TBP-associated factor subunit C, TBP-associated factor 4, Transcription initiation factor TFIID 130 kDa subunit, TAF(II)130, TAFII-130, TAFII130, Transcription initiation factor TFIID 135 kDa subunit, TAF(II)135, TAFII-135, TAFII135, TAF4, TAF2C, TAF2C1, TAF4A, TAFII130, TAFII135

All lanes: Anti-TAF4 Antibody (C-Term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: U-251 MG whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: Hela whole cell lysate. Lane 5: CCRF-CEM whole cell lysate. Lane 6: HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 110 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining TAF4 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining TAF4 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

This TAF4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1021-1062 amino acids from human TAF4.

UniProt

O00268