TAGLN2 Rabbit Polyclonal Antibody

SKU: orb704391

Description

TAGLN2 Rabbit Polyclonal Antibody

Research Area

Tumor Biomarkers, Cancer Research

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB=1:500-2000, ICC/IF=1:100
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant human TAGLN2 (1-199/199aa)
• Target: TAGLN2
• Molecular Weight: 22 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDABP0035; epididymis tissue protein Li 7e; HA1756; KIAA0120; SM22-alpha homolog; TAGL2_HUMAN; Tagln2; Transgelin 2; Transgelin-2.

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (KO Validated) TAGLN2 polyclonal Antibody, Unconjugated (orb704391) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: 293T (Human) Cell Lysate at 25 ug, Lane 2: TAGLN2 knockout (KO) 293T (Human) Cell Lysate at 25 ug, Primary: Anti-TAGLN2 (orb704391) at 1/1000 dilution, Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution, Predicted band size: 18 kD, Observed band size: 20 kD.

Sample: Lane 1: Lung (Mouse) Lysate at 40 ug, Lane 2: Placenta (Mouse) Lysate at 40 ug, Lane 3: Uterus (Mouse) Lysate at 40 ug, Lane 4: U251 (Human) Cell Lysate at 30 ug, Lane 5: A431 (Human) Cell Lysate at 30 ug, Lane 6: U87MG (Human) Cell Lysate at 30 ug, Lane 7: U2os (Human) Cell Lysate at 30 ug, Lane 8: Huvec (Human) Cell Lysate at 30 ug, Lane 9: A673 (Human) Cell Lysate at 30 ug, Primary: Anti-TAGLN2 (orb704391) at 1/1000 dilution, Anti-beta-Actin (orb500817) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 18 kD, Observed band size: 22 kD.

Quick Database Links

Gene Symbol

TAGLN2

UniProt

P37802