TBB5 Antibody

SKU: orb1440701

Description

Mouse monoclonal antibody to TBB5.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Epigenetics,Crown Antibodies;Primary Antibodies;Antibody Collections;Neuroscience;Signal Transduction;Loading Control

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF: 1:25, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:10~50
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Hamster, Other, Porcine

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b,Igκ
• Clone No.: 87CT59.3.7
• Immunogen: Recombinant Protein
• Target: TUBB
• Molecular Weight: 49671
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5

Formalin-fixed and paraffin-embedded human testis reacted with TBB5 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescent analysis of A549 cells, using TBB5 Antibody. diluted at 1：25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H.kidney section using TBB5 Antibody. diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from 293, CEM, Hela, Y79, rat PC-12 cell line, mouse brain tissue lysate (from left to right), using TBB5 Antibody. diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary Antibody. Lysates at 20 μg per lane.

Quick Database Links

Gene Symbol

TUBB

UniProt

P07437

RefSeq (Protein):NP_821133.1