TCF4 Antibody: HRP

SKU: orb413796

Description

Rabbit polyclonal antibody against TCF4 conjugated to HRP

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Cell Signaling | Epigenetics and Nuclear Signaling | Transcription | Domain Families | Hlh | Stem Cells | Signaling Pathways | Wnt / Beta Catenin Pathway

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB (1:1000); IHC (1:50)
• Reactivity: Human, Mouse
• Application Notes:
  A 1:1000 dilution of SPC-723 was sufficient for detection of TCF4 in 15 µg of mouse liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the C-terminal of Human TCF4 (500-600 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• Target: TCF4
• Molecular Weight: 71 kDa, 45 kDa
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TCF-4 antibody, MGC149723 antibody, PTHS antibody, Class B basic helix-loop-helix protein 19 antibody, bHLHb19 antibody, SEF 2 antibody, SL3 3 enhancer factor 2 antibody, SEF2 1 antibody, ITF-2 antibody, E2 2 antibody, SEF2 1A antibody, BHLHB19 antibody, MGC149724 antibody, ITF 2 antibody, Transcription factor 4 antibody, TCF 4 antibody, TCF4 antibody, SEF-2 antibody, SEF2 1B antibody, Immunoglobulin transcription factor 2 antibody, SL3-3 enhancer factor 2 antibody, SEF2 antibody, ITF2 antibody, ITF2_HUMAN antibody

Immunohistochemistry analysis using Rabbit Anti-TCF4 Polyclonal Antibody. Tissue: Kidney. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-TCF4 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Western blot analysis of Mouse liver lysate showing detection of ~71.3 kDa TCF4 protein using Rabbit Anti-TCF4 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse liver lysate. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF4 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~71.3 kDa. Other Band (s): ~45 kDa isoform.

Quick Database Links

Gene Symbol

TCF4

UniProt

P15884

NCBI Gene ID

6925

RefSeq (Protein):NP_001077431.1