TGF-Beta Antibody

SKU: orb1538404

Description

TGF-Beta Antibody

Research Area

Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: ELISA, FA, IHC, IHC-Fr, IHC-P, NeA, WB
• Dilution Range: ELISA, Func, IHC, IHC-Fr, IHC-P (10 µg/ml), Neut, WB
• Reactivity: Human
• Application Notes:
  Further information: Suitable for use in Western Blot, ELISA, Neutralization and Immunohistochemistry. Neutralization: Neutralizes TGF-beta activity in vitro in an inhibition assay of CCL/64 cell growth and the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of microinjection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo. Immunohistochemistry (formalin-fixed, paraffin-embedded, frozen): 1:1000. Has been used to demonstrate TGF-beta in ovine ovarian tissue and human breast carcinoma. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue. Western Blot: 5-20 ng/ml will allow visualization of 100 ng/lane of TGF-beta. Recognizes the dimeric (25kD) and monomeric (12.5kD) forms of TGF-beta under both non-reducing and reducing conditions respectively.

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Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: TB21
• Immunogen: TGF-b from human platelets.
• Target: TGF-Beta
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.0. PBS, pH 7.0
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

TGF-Beta