TGFBR1 Antibody (Center)

SKU: orb107025

Description

Rabbit polyclonal to TGFBR1. Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Signaling Pathways,Signaling Pathways/Apoptotic,Epigenetics,Primary Antibodies;Cancer;Cardiovascular;Metabolism;Signal Transduction;Anti-Wnt-Pathway (plus Lgr5) Antibodies

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:2000
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB43638
• Immunogen: Synthetic Peptide
• Target: TGFBR1
• Molecular Weight: 55960
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti TGFBR1 antibody, anti ALK5 antibody, anti SKR4 antibody, anti TGF-beta receptor type-1 antibody, anti TGF-beta receptor type-1 antibody, anti Activin A receptor type II-like protein kinase of 53kD antibody, anti TGF-beta receptor type-1 antibody, anti Activin receptor-like kinase 5 antibody, anti TGF-beta receptor type-1 antibody, anti Serine/threonine-protein kinase receptor R4 antibody, anti TGF-beta receptor type-1 antibody, anti TGF-beta type I receptor antibody, anti TGF-beta receptor type-1 antibody, anti Transforming growth factor-beta receptor type I antibody

Anti-TGFBR1 Antibody (Center) at 1:2000 dilution + mouse kidney lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 56 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-TGFBR1 Antibody (Center) at 1:2000 dilution + A431 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 56 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

TGFBR1

UniProt

P36897

RefSeq (Protein):NP_004603.1, NP_001124388.1