Thymidylate Synthase Antibody

SKU: orb639980

Description

It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics

Images & Validation

−

Item 1 of 3

Tested Applications	FACS, IF
Dilution Range	Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the antibody should be determined by the researcher.

Key Properties

−

Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rTYMS/1884
Immunogen	A recombinant human partial protein (amino acids 60-174) was used as the immunogen for the recombinant Thymidylate Synthase antibody.
Purity	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

−

Storage	Store the recombinant Thymidylate Synthase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives	0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Hazard Information	This recombinant Thymidylate Synthase antibody is available for research use only.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

UniProt

P04818

UniProt Details

−

No UniProt data available

Protocol Information

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

View Protocol

Immunofluorescence