TIGIT Single Domain Antibody [2C6]

SKU: orb1274205

Description

TIGIT Single Domain Antibody [2C6]

Research Area

,Immunology

Images & Validation

• Tested Applications: ELISA, FC, ICC, IF, IHC-P
• Reactivity: Human
• Application Notes:
  TIGIT antibody can be used for ELISA starting at 0.1 μg/mLimmunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. For flow cytometry at 1 μg/ml. For immunocytochemistry at 10μg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Recombinant
• Isotype: sdAb
• Clone No.: 2C6
• Immunogen: TIGIT antibody was raised against a recombinant protein corresponding to amino acids 22-141 of human TIGIT.
• Target: TIGIT
• Purification: TIGIT Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: TIGIT Antibody is supplied in PBS.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TIGIT Antibody: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM

Immunocytochemistry of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 10 μg/mL.

Immunofluorescence of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 20 μg/mL.

Immunofluorescence of TIGIT in human spleen tissue with TIGIT single domain antibody at 20 μg/mL.

Titration ELISA analysis of TIGIT sdAbs to detect recombinant TIGIT (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.

Immunohistochemistry of TIGIT in human spleen tissue with TIGIT single domain antibody at 1 μg/mL.

Flow cytometry analysis of TIGIT overexpressing HEK293 cells using TIGIT single domain antibody and control 1H4 single domain antibody at 0.05 μg/ml. Blue: Untransfected HEK293 cells. Yellow: TIGIT overexpressing HEK293 cells.

Quick Database Links

Gene Symbol

TIGIT

UniProt

Q495A1

RefSeq (Protein):NP_776160