TNF alpha Antibody

SKU: orb345255

Description

TNF Alpha antibody

Research Area

Product Categories/Products/Antibodies,Immunogen Type: Recombinant Protein

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:2,000 - 1:2,500, WB: 1:1000
• Reactivity: Porcine
• Application Notes:
  This protein A purified TNFα antibody has been tested by western blot and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.9 kDa in size corresponding to swine TNFα by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 154 amino acids of mature swine TNFα.
• Purity: This product was Protein-A purified from monospecific antiserum by chromatography. It is specific for swine TNFα protein. A BLAST analysis was used to suggest cross-reactivity with TNFα from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against TNFα from baboon based on 90% homology. Cross-reactivity with TNFα from sources other than swine has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-TNF-A antibody, rabbit anti-TNFα antibody, rabbit anti-TNF alpha antibody, cachexin, cachectin, tumor necrosis factor-alpha, TNFSF2, TNFSF1A

Quick Database Links

UniProt

P23563

RefSeq (Protein):NP_999187