TNF alpha Antibody

SKU: orb749821

Description

Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/NF-kB,Epigenetics

Images & Validation

• Tested Applications: FACS, IF, IHC-P
• Dilution Range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the TNF-a antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgM, kappa
• Clone No.: P/T2
• Immunogen: Amino acids 115-130 (NGVELRDNQLVVPSEG) from the human protein were used as the immunogen for the TNF-a antibody.
• Purity: PEG precipitation
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the TNF-a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This TNF-a antibody is available for research use only.
• Disclaimer: For research use only

Quick Database Links

UniProt

P01375