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Description
Research Area
,Apoptosis,Cancer,Chemokines & Cytokines,Growth Factors,Immunology,Innate Immunity,Obesity,Signal Transduction
Images & Validation
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| Application Notes |
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Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells). |
| Target | TNF |
| Tag | DDDDK Tag |
| Purity | > 95% (SDS-PAGE). Endotoxin level is less than 0.02EU/ μg purified protein (LAL test; Lonza). |
| Protein Sequence | The extracellular domain of human TNF-alpha (aa 85-233) is fused at the N-terminus to human ACRP30headless (aa 16-108) and a DDDDK-tag. |
Storage & Handling
−| Storage | Stable for at least 6 months after receipt when stored at -20˚°C. |
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| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Contains PBS. Reconstitute with 100 μl sterile water. |
| Concentration | 0.1mg/ml after reconstitution. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ACRP30headless:TNF-alpha, ACRP30headless:TNFSF2, ACRP30headless:Tumor Necrosis Factor-alpha
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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